| 产品介绍 | | |
| Recombinant Human CNTFis a 199 amino acid,Escherichia coli-derived recombinant protein corresponding to mature human ciliary neutotrophic factor (CTNF), a survival factor for various neuronal cell types believed to prevent the degeneration of motor axons after axotomy. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified recombinant protein - lyophilized |
| Preparation | Purified recombinant CTNF expressed inE. Coli |
| Reconstitution | Reconstitute with 0.2 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended. |
| Buffer Solution | 10mM Citric Acid + 75mM NaCl |
| Activity | The ED50as determined by the dose-dependent proliferation of human TF-1 cells is 50-150ng/ml. |
| Preservative Stabilisers | None present |
| Protein Molecular Weight | 22.7 kDa |
| Purity | >98% by SDS PAGE/HPLC |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date. |
| 存储条件 | | |
| Prior to reconstitution store at -20℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 0.2 | |
| Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
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