Recombinant Human TGF Beta 1
货号:PHP143B
规格:10 µg
价格:2661
产品类型:细胞因子添加物
品牌:Bio-Rad
| 产品介绍 | | |
| Transforming growth factor beta-1 (TGF beta 1) is a homodimeric protein that regulates the proliferation and differentiation of normal and transformed cells. TGF beta 1 is the most abundant isoform which is secreted by most cell types. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified recombinant protein - lyophilized |
| Reconstitution | Reconstitute with 100ul distilled water. Further dilutions should be made in buffer containing a carrier protein such as 0.1% BSA.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. DO NOT VORTEX. |
| Preparation | Recombinant protein prepared from CHO cells. |
| Preservative Stabilisers | None present |
| Activity | The ED50, determined by the ability of TGF beta 1 to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤0.05 ng/ml, corresponding to a specific activity of ≥2 x 107units/mg |
| Carrier Free | Yes |
| Purity | >98% by SDS PAGE and HPLC analysis |
| Protein Molecular Weight | 25.0 kDa (2x identical 112 amino acid polypeptide chains) |
| Approx. Protein Concentrations | 0.1 mg/ml after reconstitution |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date. |
| 存储条件 | | |
| Prior to reconstitution store at -20℃. Following reconstitution store at -20℃.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Functional Assays | √ | | |
| Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
技术参数 产品应用 FN
产品类型 重组蛋白
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