| 产品介绍 | | |
| Mouse anti Human CD55 antibody, clone BRIC216recognizes the CD55 antigen, a ~70 kDa glycoprotein also known as Decay Accelerating Factor (DAF). CD55 is distributed on erythrocytes and other circulating blood cells and also on cells in non-haemopoietic tissue particularly epithelium and endothelium. CD55 is also expressed at the foetal-maternal interfaces in placenta.CD55 has reduced expression on individuals with paroxysmal nocturnal haemoglobinuria. Mouse anti Human CD55 antibody, clone BRIC216 has a functional binding affinity to erythrocytes of 8.7 x 107M-1. The antigen is pronase and trypsin resistant and chymotrypsin sensitive. Mouse anti Human CD55 antibody, clone BRIC216 recognizes the consensus region 3 of the DAF molecule, which contains the functional site, and the antibody blocks the function of DAF. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | TRIS buffered saline. |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3)≤100mM Glycine |
| Immunogen | Human fibroblast cell line. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Coyne, K.E.et al.(1992) Mapping of epitopes, glycosylation sites, and complement regulatory domains in human decay accelerating factor.J Immunol. 149 (9): 2906-13.2.Loberg, R.D.et al.(2006) Inhibition of decay-accelerating factor (CD55) attenuates prostate cancer growth and survivalin vivo.Neoplasia. 8: 69-78.3.Triantafilou, M.et al.(2000) A 70 kDa MHC class I associated protein (MAP-70) identified as a receptor molecule for Coxsackievirus A9 cell attachment.Hum Immunol. 61 (9): 867-78.4.Ellison, B.S.et al.(2007) Complement susceptibility in glutamine deprived breast cancer cells.Cell Div. 2: 20. |
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