| 产品介绍 | | |
| Mouse anti Human CD58 antibody, clone BRIC5recognizes human Lymphocyte function-associated antigen 3, also known as CD58 or LFA-3. CD58 is a 250 amino acid single pass type I transmembrane glycoprotein, a member of the immunoglobulin superfamily, with a predicted molecular mass of 28.1 kDa and an apparent molecular mass of ~55-70 kDa. CD58 occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA and is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes (Makgobaet al.1989).CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.Mouse anti Human CD58 antibody, clone BRIC5 was produced in response to erythrocytes. The functional affinity of BRIC5 binding to erythrocytes is 4 x 108M-1. It reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is pronase sensitive. Mouse anti Human CD58 antibody, clone BRIC5 inhibits T cell rosetting. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Makgoba, M.W.et al.(1989) The CD2-LFA-3 and LFA-1-ICAM pathways: relevance to T-cell recognition.Immunol Today. 10 (12): 417-22.2.Shaw, S. and Johnson, J.P. (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens.Edited by Knapp, W., Dorken, B., Gilks, W.R., Rieber, E.P., Schmidt, R.E., Stein, H. and von dem Borne, A.E.G.Kr. Oxford University Press. pp 714-716.3.Grundy, J.E.et al.(1993) Increased adherence of CD2 peripheral blood lymphocytes to cytomegalovirus-infected fibroblasts is blocked by anti-LFA-3 antibody.Immunology. 78 (3): 413-20.4.Bottley, G.et al.(2005) Differential expression of LFA-3, Fas and MHC Class I on Ad5- and Ad12-transformed human cells and their susceptibility to lymphokine-activated killer (LAK) cells.Virology. 338 (2): 297-308. |
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