| 产品介绍 | | |
| Rat anti Mouse CD335 antibody, clone 29A1.4recognizes natural killer cell p46-related protein (NKp46), otherwise known as CD335, which is uniquely expressed by resting and activated natural killer (NK) cells, but no expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.It is a major NK cell lysis receptor for autologous pathogen-infected and tumor target cells during natural cytotoxicity responses. Ligands of CD335 include viral hemagglutinins (HAs) and heparan sulfate proteoglycans (HSPGs) on the surface of tumor cells.Staining with Rat anti mouse CD335 (29A1.4) is not strain specific and the antibody has been used to stain C57BL/6, SJL, CBA/CA and BALB/c strains. Clone 29A1.4 also activates NK cellsin vitro. It does not deplete NK cellsin vivo. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG - liquid |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | NKP46-IgG1 Fc fusion protein. |
| Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/10 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Ota, N.et al.(2011) IL-22 bridges the lymphotoxin pathway with the maintenance of colonic lymphoid structures during infection withCitrobacter rodentium.Nat Immunol. 12 (10): 941-8.2.Walzer, T.et al.(2007) Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46.Proc Natl Acad Sci U S A. 104: 3384-9.3.Walzer, T.et al.(2007) Natural killer cell traffickingin vivorequires a dedicated sphingosine 1-phosphate receptor.Nat Immunol. 8 (12): 1337-44.4.Luci, C.et al.(2009) Influence of the transcription factor RORgammat on the development of NKp46+ cell populations in gut and skin.Nat Immunol. 10: 75-82. |
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