| 产品介绍 | | |
| Mouse anti Rabbit GAPDH antibody, clone 6C5recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Haraet al.2006). |
| 产品详情 | | |
| Target Species | Rabbit |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | Rabbit muscle GAPDH. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Latasa, M.U.et al.(2010) Oral methylthioadenosine administration attenuates fibrosis and chronic liver disease progression in Mdr2-/- mice.PLoS One. 5: e15690.2.Haller, S.et al.(2012) Expression profiles of metabolic enzymes and drug transporters in the liver and along the intestine of beagle dogs.Drug Metab Dispos. 40 (8): 1603-10.3.Zizza, P.et al.(2012) Phospholipase A2IVα regulates phagocytosis independent of its enzymatic activity.J Biol Chem. 287: 16849-59.4.Zschemisch, N.H.et al.(2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.BMC Immunol. 13: 60. |
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