mouse anti Rat TCR alpha/beta:Low Endotoxin
货号：MCA453EL
规格：0.5 mg
价格：5228
产品类型：流式抗体
品牌：Bio-Rad
抗原： TCR alpha/beta
物种：小鼠
宿主：大鼠
抗体亚型：IgG1
克隆号：R73
荧光染料：Low Endotoxin
| 产品介绍 | | |
| Mouse anti Rat TCR alpha/beta antibody, clone R73recognizes a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative.Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colicet al.2010). |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | None present |
| Immunogen | Rat T Blasts and Erythrocytes. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20oonly.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/50 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:Low Endotoxin | MCA1209EL | F | 0.5mg |
| 文献 |
| 1.Ariki, S.G. and Roux, M.E. (1995) T lymphocyte subpopulations in the intestinal villi of immune deficient rats.In Recent Advances in Mucosal Immunology. Edited by McGhee, J., Mestecky, J., Tlaskalova, H. and Sterzl, J.2.Yoshino, N.et al.(2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110.3.Hünig, T.et al.(1989) A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes.J Exp Med. 169 (1): 73-86.4.Tomida, S.et al.(1994) Intercellular adhesion molecule-1 and leukocyte function-associated antigen-1 are involved in protection mediated by CD3+TCR alpha beta- T cells at the early stage after infection with Listeria monocytogenes in rats.Int Immunol. 6 (7): 955-61. |
技术参数 产品应用 IHC(F);IF;Flow;FN
产品类型 Monoclonal Antibody
推荐用于 IHC(F);IF;Flow;FN
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