| 产品介绍 | | |
| Mouse anti Human C3a antibody, clone 048-18.6.3.X (10-21)recognizes the ~9 kDa complement component 3a (C3a) which is present in the blood serum. C3a is generated over the course of complement activation where complement 3 (C3) convertases cleave C3 to C3a and C3b, which is further degraded into iC3b and C3dg/C3d. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.C3a mediates local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3a is expressed at significantly higher levels in colorectal cancer.Mouse anti Human C3a antibody, clone 10-21 shows minimal reactivity with other complement proteins in radioimmunoassays and haemagglutination.Mouse anti Human C3a antibody, clone 10-21 has been reported to work in western blotting applications. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from ascites |
| Buffer Solution | Borate buffered saline |
| Immunogen | Native purified human C3a. |
| Approx. Protein Concentrations | Current, batch-specific concentration 1.1 mg/ml |
| Preservative Stabilisers | 0.1% Sodium Azide (NaN3) |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 1/1500 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests/1ml |
| 文献 |
| 1.Hugli, T.E. (1975) Human anaphylatoxin (C3a) from the third component of complement. Primary structure.J Biol Chem. 250 (21): 8293-301.2.Habermann, J.K.et al.(2006) Increased serum levels of complement C3a anaphylatoxin indicate the presence of colorectal tumors.Gastroenterology. 131 (4): 1020-9; quiz 1284. |
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