mouse anti CLOSTRIDIUM DIFFICILE TOXIN A
货号：MCA2597
规格：0.1 mg
价格：5950
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Clostridium difficile Toxin A
物种：Bacterial
宿主：小鼠
抗体亚型：IgG1
克隆号：5155
荧光染料：Purified
| 产品介绍 | | |
| Mouse antiClostridium dificiletoxin A antibody, clone 5155recognizesClostridium difficiletoxin A. No reaction is observed withClostridium difficiletoxin B, tetanus toxoid,Pseudomonas aeruginosaandYersinia enterocoliticaClostridium difficileis a gram-positive motile bacterium which is the leading cause of diarrhoea in developed countries. The incidence of disease in humans varies greatly with age, spore density and the administration of antibiotics.Toxin A (TcdA) is a large exotoxin which is an essential virulence factor forC. difficileinfection. TcdA has a molecular mass of ~308 kDa and is a glucosyltransferase, which irreversibly inactivates the Ras family of small GTPases to disrupt many important signalling pathways. |
| 产品详情 | | |
| Target Species | Bacterial |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Approx. Protein Concentrations | IgG concentration 0.1mg/ml |
| Fusion Partners | Cells from immunised mice were fused with cells of the mouse Sp-2/0-Ag14 myeloma cell line. |
| Immunogen | Clostridium difficiletoxin A. |
| Preservative Stabilisers | 0.1%Sodium Azide (NaN3) |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Voth, D.E. & Ballard, J.D. (2005)Clostridium difficiletoxins: mechanism of action and role in disease.Clin Microbiol Rev. 18 (2): 247-63. |
技术参数 产品应用 ELISA
产品类型 单克隆抗体
推荐用于 ELISA
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