Mouse anti Human CD49b antibody, clone 16B4
货号：MCA2025PE
规格：100 Tests
价格：3188
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD49b
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：16B4
荧光染料：PE
| 产品介绍 | | |
| Mouse anti Human CD49b antibody, clone 16B4recognizes human CD49b, also known as integrin α2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid ~160 kDa single pass type-1 transmembrane glycoprotein possessing multipleFG-GAPrepeats and a singleVWFAdomain. Mouse anti human CD49b antibody, clone 16B4 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125surface labeled human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilizedPurified human beta 1 preparation from HT1080 fibrosarcoma cell extract |
| Reconstitution | Reconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Immunogen | Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Rat IgG2a Negative Control:RPE | MCA1212PE | F | 100 Tests |
| 文献 |
| 1.Fitter, S.et al.(1999) Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion.Biochem J. 338 ( Pt 1): 61-70.2.Sincock, P.M.et al.(1999) PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.J Cell Sci. 112 ( Pt 6): 833-44.3.Eaton, C.L.et al.(2010) Evaluation of the frequency of putative prostate cancer stem cells in primary and metastatic prostate cancer.Prostate. 70 (8): 875-82.4.Gassmann, P.et al. (2009) CXCR4 regulates the early extravasation of metastatic tumor cells in vivo.Neoplasia. 11: 651-61. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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