mouse anti human CD49c:FITC
货号：MCA1948F
规格：0.1 mg
价格：2865
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD49c
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
克隆号：17C6
荧光染料：FITC
| 产品介绍 | | |
| Mouse anti Human CD49c antibody, clone 17C6recognizes CD49c, a ~150 kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is very weakly expressed on peripheral blood leucocytes and is not found on platelets. Epithelial cells and endothelial cells express significant levels of CD49c.Mouse anti Human CD49c antibody, clone 17C6 is routinely tested by flow cytometry on A431 epithelial cell line. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
Immunogen | CD49c transfected cell line. |
| Approx. Protein Concentrations | IgG concentration 0.1mg/ml |
| Fusion Partners | Cells from immunised Balb/c mice were fused with cells of the NS2 mouse myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
FITC | 490 | 525 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 5ul of the suggested working dilution to label 106cells in 100ul. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:FITC | MCA928F | Flow | 100 Test |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Human Seroblock | BUF070A | F | 50 Test |
| Human Seroblock | BUF070B | F | 200 Test |
| 文献 |
| 1. Nbaum, G.et al.(2006) Peptide p277 of HSP60 signals T cells: inhibition of inflammatory chemotaxis.Int Immunol. 18: 1413-9.2. Steinberg, F.et al.(2012) SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways.J Cell Biol. 197 (2): 219-30.3. Zanin-Zhorov, A.et al.(2003) T cells respond to heat shock protein 60 via TLR2: activation of adhesion and inhibition of chemokine receptors.FASEB J. 17: 1567-9. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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