Mouse anti Sheep GM-CSF antibody, clone 8D8
货号：MCA1923
规格：0.25 mg
价格：4038
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：GM-CSF
物种：绵羊
宿主：小鼠
抗体亚型：IgG1
克隆号：8D8
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Sheep GM-CSF antibody, clone 8D8recognizes both recombinant and native ovine granulocyte / macrophage colony-stimulating factor (GM-CSF), also known as Colony stimulating factor (CSF).GM-CSF is one of several cytokines that regulate the survival, growth and differentiation of haemopoietic cells. GM-CSF is secreted from a range of cells including macrophages, T-cells, endothelial cells and fibroblasts in response to antigen, LPS or mitogen stimulation.Mouse anti Sheep GM-CSF antibody, clone 8D8 may be used as a capture antibody in ELISA assays with Mouse anti Sheep GM-CSF antibody, clone 3C2 (MCA1924) as a detection reagent for the evaluation of ovine GM-CSF.Mouse anti Sheep GM-CSF antibody, clone 8D8 has been reported to neutralize the activity of both recombinant and native ovine GM-CSF (Entricanet al.1996). Removal of the preservative is recommended prior to use in functional assays. |
| 产品详情 | | |
| Target Species | Sheep |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Immunogen | CHO expressed recombinant ovine GM-CSF. |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of the NS0 myeloma cell line. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Acknowledgements | This reagent was evaluated at the Moredun Research Institute and The Roslin Institute at the University of Edinburgh as part of ‘The route to identification of immunological correlates of protection in ruminants’ Industrial Partnership Award funded by BBSRC (grant numbers BB/I019863/1; BB/I020519/1) with the support of Bio-Rad. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Entrican, G.et al.(1996) Development of a sandwich ELISA for ovine granulocyte/macrophage colony-stimulating factor.Vet Immunol Immunopathol. 50 (1-2): 105-15.2.Pedersen, L.G.et al.(2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species.Vet Immunol Immunopathol. 88 (3-4): 111-22.3.Scott, J.L.et al.(2007) Granulocyte-macrophage colony stimulating factor and interleukin-8 in the reproductive tract of ewes following oestrus and mating.Reprod Fertil Dev. 19: 585-93. |
技术参数 产品应用 Elisa;WB
产品类型 Monoclonal Antibody
推荐用于 Elisa;WB
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