| 产品介绍 | | |
| Rat anti Mouse MARCO antibody, clone ED31recognizes the murine cell surface antigen designated MARCO (Macrophage receptor with collagenous structure), which is a member of the class A scavenger receptor family.MARCO is expressed by distinct populations of macrophages in the spleen and lymph nodes, but is rapidly induced on macrophages in other tissues (e.g. Liver Kupffer cells) during infection or LPS treatment.Rat anti Mouse MARCO antibody, clone ED31 binds to the C-terminal cysteine rich domain of MARCO, and has been shown to block ligand binding. |
| 产品详情 | | |
Target Species | Mouse |
| Product Form | PurifiedIgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
| Carrier Free | Yes |
| Immunogen | CHO cells expressing mouse MARCO. |
| Approx. Protein Concentrations | IgG concentration 1 mg/ml |
| Fusion Partners | Spleen cells from immunised rats were fused with cells of the mouse SP2/0 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | x | | |
| Immunofluorescence | √ | | |
| Immunohistology - Frozen | √ | 1/200 | 1/400 |
| Western Blotting | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 数据 |
| Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MCA1849) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). High power.Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MCA1849), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 (MCA1108), red in B. C is the merged image wiith nuclei counterstained blue using DAPI. high power |
| 文献 |
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