| 产品介绍 | | |
| Mouse anti Human CD117 antibody, clone 104D2recognizes the human CD117 cell surface antigen, a 145 kDa glycoprotein also known as c-kit. CD117 is expressed by some haematopoietic stem cells (approx. 70% of CD34+ve cells in normal bone marrow are CD117+ve), some acute myeloid leukaemias and by mast cells.Functionally CD117 is a receptor for stem cell factor, and has receptor tyrosine kinase activity.Mouse anti Human CD117 antibody, clone 104D2 is non-neutralizing. |
| 产品详情 | | |
Target Species | Mouse |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Cynomolgus monkey | √ |
| Bovine | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
| Immunogen | MOLM-1 megakaryocyte cell line |
| Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
Regulatory | For research purposes only |
Guarantee | Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date. |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/2 | 1/20 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
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| 同型 |
| Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
| 文献 |
| 1. Broudy, V.C.et al.(1998) Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer.Blood. 91 (3): 898-906.2. Yoshino, N.et al.(2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110.3. Jayapal, M.et al.(2006) Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcepsilonRI-aggregation reveals a complex network of genes involved in inflammatory responses.BMC Genomics. 7: 210.4. Randall, V.A.et al.(2008) Stem cell factor/c-Kit signalling in normal and androgenetic alopecia hair follicles.J Endocrinol. 197: 11-23.5. Venkata, N.S.G.et al.(2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.PLoS One. 13 (2): e0192244. |
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