| 产品介绍 | | |
| Mouse anti Human MCP-3 antibody, clone h.mcp.3recognizes human C-C motif chemokine 7, also known as Monocyte chemoattractant protein 3, MCP-3, CCL7, NC28 or Small-inducible cytokine A7. MCP-3 is a 99 amino acid (including signal peptide sequence) ~9kDa chemotactic glycoprotein for monocytes and eosinophils. No cross reactivity is observed with MCP-1, MCP-2 or other structurally related chemokines. |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG - lyophilized |
| Reconstitution | Reconstitute with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use. |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
| Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
| Carrier Free | Yes |
| Immunogen | Recombinant human MCP-3 (Macrophage Chemoattractant Protein-3). |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised C3H/HeJ mice were fused with cells of a mouse myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/1000 |
| Functional Assays | √ | | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1. Al-Bannawi, A.et al.(2011)Chlamydia pneumoniaeinduces chemokine expression by platelets in patients with atherosclerosis.Med Princ Pract. 20 (5): 438-43. |
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