| 产品介绍 | | |
| Mouse anti Bovine CD26 antibody, clone CC69recognizes bovine Dipeptidyl peptidase 4, also known as CD26, WC10, Adenosine deaminase complexing protein or Activation molecule 3 (Leeet al.2001). Bovine CD26 is a 765 amino acid single pass type II transmembrane glycoprotein which can be cleaved between residues 37-38 to release a soluble form of the protein. CD26 can exist as a monomer, forms a homodimer for optimal dipeptidase activity or it can form a heterodimer with seprase.CD26 is expressed by a subpopulation of CD2+ T cells, dendritic cells and weakly by most B cells and is also seen on some non-haematopoietic cells, especially gut epithelium. Myeloid cells and the majority of WC1+veT cells do not express CD26. |
| 产品详情 | | |
| Target Species | Bovine |
| Product Form | Purified IgG conjugated to Alexa Fluor® 647 - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Human monocytes |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®647 | 650 | 665 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 同型对照 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
| 文献 |
| 1.Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).Vet Immunol Immunopathol. 39 (1-3): 25-47.2.Naessens, J.et al.(1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.Vet Immunol Immunopathol. 39 (1-3): 283-90.3.Epardaud, M.et al.(2004) Enrichment for a CD26hi SIRP- subset in lymph dendritic cells from the upper aero-digestive tract.J Leukoc Biol. 76: 553-61.4.Contreras, V.et al.(2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.J Immunol. 185: 3313-25. |
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