Phusion™ Plus PCR Master Mix/Phusion™ Plus高保真PCR预混液
货号：F631XL,F631L,F631S
规格：2000 x 50 µL reactions,500 x 50 µL reactions,100 x 50 µL reactions
价格：16735,4647,1182
产品类型：核酸合成
品牌：Thermo Fisher
| Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs. The master mix format simplifies reaction setup with minimal pipetting steps and retains all features of the stand-alone format.Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus PCR Master Mix to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance. Its novel reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C. This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs. Furthermore, with Phusion Plus PCR Master Mix and its universal annealing feature, different PCR reactions can be cycled together using the same protocol.Phusion Plus PCR Master Mix is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature. Enzyme activity is fully restored after the initial denaturation step.Applications• High-fidelity PCR• Cloning• Template generation for sequencing• GC-rich PCR• Long-range PCR (up to 20 kb)• Mutagenesis• High-throughput PCR |
优点： |
| -High fidelity—provides >100X greater sequence accuracy than Taq polymerase-Convenient setup—enables a universal annealing temperature of 60°C in PCR-Enhanced specificity—hot-start modification reduces non-specific amplification and primer degradation-Benchtop stability—automation enabled due to 24-hour stability of assembled reactions-High inhibitor tolerance—works with DNA of suboptimal purity |
相关产品： |
▪Phusion Plus Green PCR Master Mix/高保真绿色PCR预混液 ▪Phusion Plus PCR Master Mix/高保真PCR预混液▪Phusion™ Plus DNA Polymerase/Phusion™ Plus DNA高保真聚合酶 |
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