RevertAid™ H Minus First Strand cDNA Synthesis Kit, with DNase I/RevertAid™第一链cDNA合成试剂盒（含DNase I）
货号：K16315,K16325
规格：20 reactions,100 reactions
价格：0,1687
产品类型：核酸合成
品牌：Thermo Fisher
| The Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit (with DNase I) is a complete system for efficient synthesis of first-strand cDNA from RNA templates. The kit includes recombinant endonuclease (DNase I) to remove contaminating genomic DNA from RNA preps.For reverse transcription the kit uses RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA. |
优点： |
| - Integrated genomic DNA removal step- High yields of full-length first-strand cDNA up to 13 kb- Increased reaction temperatures in the range of 42 to 55°C- Supplied with the recombinant RiboLock RNase Inhibitor- Complete kit—oligo(dT)18 and random hexamer primers included with the kit |
组成成分： |
| ▪1 x 25 µL RevertAid H Minus Reverse Transcriptase (200 U/µL)▪1 x 25 µL RiboLock RNase Inhibitor (20 U/µL)▪1 x 150 µL 5X Reaction Buffer▪1 x 50 µL dNTP Mix (10 mM)▪1 x 25 µL Oligo(dT)18 Primer (100 µM)▪1 x 25 µL Random Hexamer Primer (100 µM)▪1 x 20 µL Control GAPDH RNA (0.05 µg/µL)▪1 x 20 µL Forward GAPDH Primer (10 µM)▪1 x 20 µL Reverse GAPDH Primer (10 µM)▪2 x 1.25 mL water, nuclease-free▪1 x 20 µL DNase I, RNase-free (1 U/µL)Store at -20°C. |
相关产品： |
| ▪RevertAid cDNA第一链合成试剂盒（含DNase I）▪RevertAid H Minus第一链cDNA合成试剂盒（含DNase I）▪RevertAid 预混液（含DNase I） |
技术参数 产品应用 • First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis
QQ:1749072012