| 产品介绍 | | |
| Mouse anti Chicken CD3, clone CT-3recognizes the chicken CD3 cell surface antigen expressed by T cells (Chenet al.1986). The antibody also co-precipitates a ~90 kDa heterodimer from digitonin solubilised T cell lysates, which can be reduced to two polypeptides of ~40 kDa and ~50 kDa.In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow. |
| 产品详情 | | |
Target Species | Chicken |
| Product Form | Purified IgG - liquid |
| Reconstitution | Reconstitute with 1 ml distilled water |
Preparation | Purified IgG prepared from ascites |
BufferSolution | Borate buffered saline |
Preservative Stabilisers | None present |
| Immunogen | Chicken thymocytes and Ig -ve peripheral blood mononuclear cells. |
| Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
| Fusion Partners | Lymph node cells from immunised Balb/c mice were fused with cells from the mouse P3-X63Ag8.653 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | 1/50 |
| Immunohistology - Frozen | √ | | |
| Immunoprecipitation | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 50ul of the suggested working dilution to label 106cells in 100ul.
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| 数据 |
| Figure A. FITC conjugated Mouse anti Chicken CD8 (MCA2166F) and Mouse IgG1 isotype control (MCA928) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A). Figure B. Mouse anti Chicken CD3 (MCA1473) followed with Rabbit F(ab')2anti Mouse IgG:RPE (STAR12A) and FITC conjugated Mouse anti Chicken CD8 (MCA2166F). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer. |
| 文献 |
| 1. Chen, C.L.et al.(1986) Identification of a T3/T cell receptor complex in chickens.J Exp Med. 164 (1): 375-80.2. Mukamoto, M. & Kodama, H. (2000) Regulation of early chicken thymocyte proliferation by transforming growth factor-beta from thymic stromal cells and thymocytes.Vet Immunol Immunopathol. 77 (1-2): 121-32.3. Chaussé, A.M.et al.(2011) Expression of toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to salmonella carrier state.Infect Immun. 79: 3445-54.4. Balic, A.et al.(2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.Development. 141: 3255-65.5. Tang, Y.et al.(2020) Immune Modulation and the Development of Fowl Typhoid: A Model of Human Disease?Pathogens. 9 (10)Oct 15 [Epub ahead of print]. |
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