| 产品介绍 | | |
| Mouse anti PCNA antibody, clone PC10recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Liet al.1995) through its interaction withFEN1(Wuet al.1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravoet al.1987).PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti PCNA antibody, clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse (Parket al.2008), chicken (Franz-Odendaal 2008) and abalone (Harriset al.2006). |
| 产品详情 | | |
Target Species | Rat |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Vertebrates | Expected from Sequence |
| Invertebrates | Expected from Sequence |
| Ferret | √ |
| Chicken | √ |
| Rabbit | √ |
Xenopus | √ |
| Mouse | √ |
| Horse | √ |
| Sheep | √ |
| Dog | √ |
| Cat | √ |
| Cynomolgus monkey | √ |
| Rhesus Monkey | √ |
| Hamster | √ |
| Atlantic Salmon | √ |
Human | √ |
| Bearded Dragon | √ |
| Corn Snake | √ |
| Nile Crocodile | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Carrier Free | Yes |
| Immunogen | Rat PCNA made in the protein A expression vector pR1T2T |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry 1 | √ | Neat | 1/100 |
| Immunohistology - Frozen | | | |
| Immunohistology - Paraffin | | 1/50 | 1/100 |
| Immunoprecipitation | | | |
| Western Blotting | | | 1/1000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product CodeBUF09) for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul.
- ImmunohistologyThis product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat pre-treatment prior to staining of paraffin sections.
- Histology Positive Control TissueTonsil
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| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG2a Negative Control | MCA1210 | Flow | 100 Tests |
| 数据 |
| Multiplex staining ofHeLawhole cell lysate with Mouse anti PCNA antibody, clone PC10 (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight 680 (STAR117D680GA) shown in red and Rabbit anti GAPDH(AHP1628)detected with Sheep anti Rabbit IgG:DyLight 488 shown in green. |
| 文献 |
| 1. Fenton, M.et al.(2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.Arterioscler Thromb Vasc Biol. 21: 220-6.2. Park, J.H.et al.(2008) Gastric lesions and immune responses caused by long-term infection with Helicobacter heilmannii in C57BL/6 mice.J Comp Pathol. 139: 208-17.3. Izhak, L.et al.(2012) Dissecting the autocrine and paracrine roles of the CCR2-CCL2 axis in tumor survival and angiogenesis.PLoS One. 7: e28305.4. Di-poï, N. & Milinkovitch, M.C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.Sci Adv. 2 (6): e1600708.5. Guzera, M.et al.(2016)In VitroInfluence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.PLoS One. 11 (5): e0154429.6. Khlebnikov, V. V.et al.(2015) Developmental Characteristics of the Hypothalamo-Hypophyseal-Adrenal System in Chronic Heterotypical StressNeuroscience and Behavioral Physiology. 46 (1): 100-5.7. Nakatsuka, M. & Kumabe, S (2018) Histological Alterations from Condyle Repositioning with Functional Appliances in Rats.J Clin Pediatr Dent. 42 (5): 391-7.8. Falbo, L.et al.(2020) SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development.Nat Commun. 11 (1): 1345. |
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