| 产品介绍 | | |
| Mouse anti Human Bcl-2 antibody, clone 100recognizes the human Apoptosis regulator Bcl-2 oncoprotein, a 239 amino acid ~25kDa integral single pass membrane protein containing 4BH motifswhich lies within the cell rather than on the cell surface. The protein is localised in the outer mitochondrial membrane and plays a role in the inhibition of apoptosis.Mouse anti Human Bcl-2, clone 100 reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-haematopoietic tissues few cells are stained (Krajewskiet al.1995). Mouse anti Human Bcl-2 antibody, clone 100 reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma (Reed 1997).Mouse anti human Bcl-2, clone 100 has been successfully used to demonstrate Bcl-2 in formalin fixed, paraffin embedded tissues of radioresistant squamous cell carcinoma by immunohistochemistry (Condonet al.2002). |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
| Carrier Free | Yes |
| Immunogen | Synthetic peptide, amino acids 41-54 of the Bcl-2 protein |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry1 | √ | 1/50 | 1/100 |
| Immunohistology - Frozen | √ | 1/20 | 1/40 |
| Immunohistology - Paraffin2 | √ | 1/20 | 1/40 |
| Immunoprecipitation | √ | | |
| Western Blotting | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™(Product CodeBUF09) for this purpose.
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Clickhereto learn how we validate our PrecisionAb range.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul.
- Histology Positive Control TissueHuman tonsil
|
| 数据 |
| Staining of paraffin embedded human tonsil with Mouse anti Human Bcl-2 (MCA1550).Western blot analysis of Bcl-2 CRISPR knockout HeLa (Bcl-2 KO) and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti Bcl-2 antibody (MCA1550) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 600 second and (B) 30 second exposure. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control | MCA928 | Flow | 100 Tests |
| 文献 |
| 1. Hockenbery, D.et al.(1990) Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death.Nature. 348 (6299): 334-6.2. Chylicki, K.et al.(2000) Characterization of the molecular mechanisms for p53-mediated differentiation.Cell Growth Differ. 11: 561-71.3. Shenker, B.J. ,i>et al. (2001) Induction of apoptosis in human T cells by Actinobacillus actinomycetemcomitans cytolethal distending toxin is a consequence of G2 arrest of the cell cycle.J Immunol. 167: 435-41.4. Iannone, F.et al.(2005) Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism.Ann Rheum Dis. 64: 217-21.5. Iscache, A.L.et al.(2011) Effects of BCL-2 over-expression on B cells in transgenic rats and rat hybridomas.Int Immunol. 23 (10): 625-36.6. Valgimigli, M.et al.(2003) Serum from patients with acute coronary syndromes displays a proapoptotic effect on human endothelial cells: a possible link to pan-coronary syndromes.Circulation. 107: 264-70.7. Berrieman, H.K.et al.(2005) The expression of Bcl-2 family proteins differs between nonsmall cell lung carcinoma subtypes.Cancer. 103: 1415-9.8. Fimognari, C.et al.(2002) Growth inhibition, cell-cycle arrest and apoptosis in human T-cell leukemia by the isothiocyanate sulforaphane.Carcinogenesis. 23: 581-6. |
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