Rat anti human CARTILAGE OLIGOMERIC MATRIX protein
货号：MCA1455G
规格：0.2 mg
价格：3120
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Cartilage Oligomeric Matrix Protein
物种：人
宿主：大鼠
抗体亚型：IgG2a
克隆号：MA37C94 (HC484D1)
荧光染料：Purified
| 产品介绍 | | |
| Rat anti Human cartilage oligomeric matrix protein antibody, clone MA37C94recognizes human cartilage oligomeric matrix protein (COMP), otherwise known as thrombospondin-5 (TSP-5). COMP is a 757 amino acid matrix glycoprotein bearing four EGF-like domains, a single TSP C-terminal domain and eight TSP type-3 repeats (UniprotP49747). Defects in the COMP gene can lead to the presence of pseudoachondroplasia or multiple epiphyseal dysplasia (Poseyet al.2014).Rat anti Human cartilage oligomeric matrix protein, clone MA37C94 recognizes an epitope located in the central portion of the molecule and has been described as suitable for use in western blotting (Gagarinaet al.2008) and immunohistochemistry (Grigoriadiset al.2006). |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
| Carrier Free | Yes |
| Immunogen | Human cartilage derived COMP |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
| Immunocytochemistry-Paraffin | √ | | |
| Immunohistology - Frozen | √ | | |
| Immunoprecipitation | √ | | |
| Western Blotting | √ | 1/200 | 1/2000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 数据 |
| Western blot analysis of recombinant Human cartilage oligomeric matrix protein probed with Rat anti Human Cartilage Oligomeric Matrix Protein (MCA1455G) followed by HRP conjugated Goat anti Rat IgG (STAR72) |
| 文献 |
1. Kobayashi, M.et al.(2016) Cartilage Oligomeric Matrix Protein Increases in Photodamaged Skin.J Invest Dermatol. 136 (6): 1143-9.2. Milz, S.et al.(2005) An immunohistochemical study of the extracellular matrix of the tarsal plate in the upper eyelid in human beings.J Anat. 206 (1): 37-45.3. Zilkens, C.et al.(2010) Spinning around or stagnation - what do osteoblasts and chondroblasts really like?Eur J Med Res. 15 (1): 35-43.4. Milz, S.et al.(2007) An immunohistochemical study of the triangular fibrocartilage complex of the wrist: regional variations in cartilage phenotype.J Anat. 211 (1): 1-7.5. Inui, S.et al.(2011) Identification and characterization of cartilage oligomeric matrix protein as a novel pathogenic factor in keloids.Am J Pathol. 179 (4): 1951-60.6. Viehöfer, A.F.et al.(2015) The molecular composition of the extracellular matrix of the human iliolumbar ligament.Spine J. 15 (6): 1325-31.7. Nemoto, M.et al.(2013) Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration.J Equine Sci. 24 (2): 17-24.8. Gong, S.D.et al.(2019) Elevated plasma cartilage oligomeric matrix protein (COMP) level are associated with the progression of non-traumatic osteonecrosis of femoral head.Clin Chim Acta. 490: 214-21.
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产品应用 IHC;ELISA;WB;IP
产品类型 Monoclonal Antibody
推荐用于 IHC;ELISA;WB;IP
QQ:1749072012