| 产品介绍 | | |
| Mouse anti GST antibody, clone vpg66reacts with Glutathione-S-transferase |
| 产品详情 | | |
| Product Form | Purified IgG conjugated to Biotin - liquid |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
| Immunogen | GST fromSchistosoma japonicum. |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
| Western Blotting | √ | 1/100 | 1/500 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| Human Seroblock | BUF070B | F | 200 Test |
| 数据 |
| Western blot analysis of GST ubiquitin recombinant protein probed with Mouse anti glutathione-S-transferase (MCA1352) followed by F(ab')2Rabbit anti mouse IgG:HRP (STAR13B) |
| 文献 |
| 1. Harris, M.P. & Neil, J.C. (1994) Myristoylation-dependent binding of HIV-1 Nef to CD4.J Mol Biol. 241 (2): 136-42.2. Blüggel, M.et al.(2011) Toward protein biomarkers for allergy: CD4+ T cell proteomics in allergic and nonallergic subjects sampled in and out of pollen season.J Proteome Res. 10 (4): 1558-70.3. Shelton, H. & Harris, M. (2008) Hepatitis C virus NS5A protein binds the SH3 domain of the Fyn tyrosine kinase with high affinity: mutagenic analysis of residues within the SH3 domain that contribute to the interaction.Virol J. 5: 24.4. Foster, T.L.et al.(2011) Cyclophilin A interacts with domain II of hepatitis C virus NS5A and stimulates RNA binding in an isomerase-dependent manner.J Virol. 85: 7460-4.5. Elfgang, C.et al.(1999) Evidence for specific nucleocytoplasmic transport pathways used by leucine-rich nuclear export signals.Proc Natl Acad Sci U S A. 96: 6229-34.6. Davis, M.P. (2008) Recombinant VP4 of human rhinovirus induces permeability in model membranes.J Virol. 82: 4169-74.7. Heger, P.et al.(1998) Multimer formation is not essential for nuclear export of human T-cell leukemia virus type 1 Rex trans-activator protein.J Virol. 72: 8659-68.8. Lischka, P.et al.(2001) A novel transferable nuclear export signal mediates CRM1-independent nucleocytoplasmic shuttling of the human cytomegalovirus transactivator protein pUL69.EMBO J. 20: 7271-83. |
QQ:1749072012