| 产品介绍 | | |
| Rat anti Dog CD90, clone YKIX337.217recognizes the canine Thy-1 cell surface antigen, also known as CD90. CD90 is a 20-30 kDa glycoprotein expressed by peripheral T lymphocytes, monocytes, thymocytes and vascular endothelium in dogs.Rat anti Dog CD90, clone YKIX337.217 has been demonstrated to deplete T cells when administeredin vivo(Watsonet al.1993), and to block Con A driven T cell proliferationin vitro.Rat anti Dog CD90, clone YKIX337.217 is a useful clone for the immunophenotyping of cells and discrimination between a wide variety of lymphoproliferative disorders in dogs (Adamet al.2009). |
| 产品详情 | | |
| Target Species | Dog |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Immunogen | Con A stimulated canine T cells. |
| Approx. Protein Concentrations | IgG concentration 1 mg/ml |
| Fusion Partners | Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | 1/50 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
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| 文献 |
| 1.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.2.Carneiro, C.M.et al.(2007) Differential impact of metacyclic and blood trypomastigotes on parasitological, serological and phenotypic features triggered during acute Trypanosoma cruzi infection in dogs.Acta Trop. 101: 120-9.3.Kang, J.W.et al.(2008) Soluble factors-mediated immunomodulatory effects of canine adipose tissue-derived mesenchymal stem cells.Stem Cells Dev. 17: 681-93.4.Sanchez, M.A.et al.(2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.Am J Trop Med Hyg. 70: 618-24. |
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