| 产品介绍 | | |
| This test employs the property of Annexin V to bind to the membrane phospholipid phosphatidylserine (PS) in the presence of Ca2+. PS is exposed at the cell surface during the early stages of apoptosis. Detection of PS is a very sensitive method for detecting cells entering apoptosis, at a time point considerably ahead of nuclear changes such as DNA degradation.The conjugation protocol used to prepare this product has not changed the native phospholipid binding properties of Annexin V. This protocol is designed to measure apoptosis easily and quickly in a sample of suspended cells. |
| 产品详情 | | |
| Preservative Stabilisers | 0.09%Sodium Azide (NaN3)1%Bovine Serum Albumin |
| Reagents in the Kit | Annexin V:PE | 200 Tests |
| 7-AAD | 100 Tests |
| 10X Binding Buffer | 100 ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | Neat |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Instructions For Use1) Dilute the10X Binding Buffer to 1X in distilled water (1 ml Binding Buffer + 9 ml distilled water).2) Wash cells once in PBS by gentle shaking or pipetting up and down. Then wash once in 1X Binding Buffer.3) Resuspend cells in 1X Binding Buffer, adjusting to a cell density of 1-5 x 106cells/ml.4) Add 5 μl of PE conjugated Annexin V to 100 μl of the cell suspension.5) Mix and incubate for 10 to15 minutes at room temperature.6) Wash cells in 1X Binding Buffer.7) Resuspend cells in 200 μl of 1X Binding Buffer.8) Add 5 μl of 7-AAD Viability Staining Solution and incubate for 5 minutes at room temperature.9) Analyse by flow cytometry within 4 hours. Store at 2-8°C in the dark. |
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