Rabbit anti 4EBP1 antibody
货号：AHP993
规格：0.1 mg
价格：4990
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：4EBP1
物种：其它
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti 4EBP1 antibodyrecognizes an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation.The association between 4EPB1 and eukaryotic translation initiation factor 4E (eIF-4E) inhibits the binding of eIF-4E with p220, thereby preventing the formation of a functional eIF-4E complex, essential for mRNA translation. Phosphorylation of 4EBP1 following stimulation by mitogens such as insulin, results in the release of eIF-4E from 4EBP1, thereby enabling the translation complex to assemble.Rabbit anti 4EBP1 antibody (AHP993) has been reported as suitable for use in IHC on mouse 3T3 cell cytospins. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Buffer Solution | Phosphate buffered saline |
| Antiserum Preparation | Antisera to human 4EBP1 (CT) were raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG prepared by affinity chromatography. |
| Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
| Immunogen | A 14 amino acid peptide located near human 4EBP1 carboxy-terminus. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining alquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 2.5 | 5.0ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.Hara, K.et al.(1997) Regulation of eIF-4E BP1 phosphorylation by mTOR.J Biol Chem. 272 (42): 26457-63.2.Haghighat, A.et al.(1995) Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E.EMBO J. 14 (22): 5701-9. |
技术参数 产品应用 wb
产品类型 多克隆抗体
推荐用于 wb
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