产品介绍 |
| Rabbit anti Cdc2 antibodyrecognizes cell division control protein 2 homolog (Cdc2) also known as cyclin-dependent kinase 1 (CDK1) and p34 protein kinase. Cdc2, a serine/threonine kinase, plays a crucial role in regulation of the eukaryotic cell cycle by phosphorylating proteins involved in DNA replication and other important cell cycle progression processes (Enserink and Kolodner 2010). Binding of cyclins to Cdc2 is essential for its regulation, and the kinase only becomes activated once bound to cyclins (Enserink and Kolodner 2010). Cyclin binding has also been reported to play a role in substrate recruitment (Enserink and Kolodner 2010).Cdc2 activity is highly regulated through phosphorylation by the serine/threonine protein kinase Wee1 (Harveyet al.2005). This phosphorylation results in Cdc2 inactivation and consequently, cell cycle arrest at the G2/M transition stage (Harveyet al.2005). Wee1 phosphorylation is counteracted by the Cdc25 phosphatase, which dephosphorylates Cdc2 thereby enabling entry into mitosis (Harveyet al.2005). |
产品详情 |
Target Species | Human |
Species Cross-Reactivity | Target Species Cross Reactivity |
| Mouse √ |
| Rat √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography |
AntiserumPreparation | Antiserum to Cdc2 was raised by repeated immunisation of rabbits with highly purified antigen |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.1% Sodium Azide (NaN3)0.2% Gelatin |
Immunogen | Peptide mapping to the carboxy terminus of human Cdc2 |
Approx. Protein Concentrations | IgG concentration 0.2 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | 1/400 | 4ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- Rabbit anti Cdc2 recognizes a band of approximately 34 kDa when run under reducing conditions
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