产品介绍 |
| Goat anti Wnt-3 antibodyrecognises both human and mouse proto-oncogene Wnt-3, a member of the Wnt family, and ligand of the frizzled family of seven transmembrane receptors.Wnt-3, otherwise known as Int-4, is involved in cell fate decisions and patterning during embryogenesis, cell signalling during neural tube development, and implicated in the tumorogenesis of several cancers including breast, lung, hepatocellular(HCC), and gastric carcinoma.Over-expression of Wnt-3 playes a pivotal role in the resistance of HER2-overexpressing breast tumors to the breast cancer treatment drug Trastuzumab, also known as Herceptin (Wuet al.2012). The effects of Wnt-3 over-expression included the transactivated expression of EGFR (epidermal growth factor receptor) an increased expression of B-catenin within the nucleus, an increase in N-cadherin and the neural crest transcription factor SLUG, as well as promoting an EMT (epithelial to mesenchymal transition) response.Goat anti Wnt-3 polyclonal antibody exhibits strong fibrous staining in myoepithelial cells of human breast tissue in paraffin sections.Goat anti Wnt-3 polyclonal antibody stains mouse hippocampal primary astrocytes in ICC/IF (Okamotoet al.2011). |
产品详情 |
Target Species | Human |
Species Cross-Reactivity | Target Species Cross Reactivity |
| Mouse √ |
| Dog Expected from Sequence |
| Rat Expected from Sequence |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to Wnt-3 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography using the immunizing peptide |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin |
Immunogen | Synthetic peptide sequence C-GRGHNTRTEKRKEK from the internal region of Wnt-3 (NP_110380.1). |
Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1:16000 |
Immunofluorescence | √ | | |
Immunohistology - Paraffin1 | √ | 3.0 | 5.0ug/ml |
Western Blotting | √ | 1.0 | 3.0ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Human breast
- Western Blotting
- AHP2268 detects a band of approximately 40kDa in HEK293 cell lysates
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文献 |
| Further Reading1.Wu, Y.et al.(2012) Expression of Wnt3 activates Wnt/β-catenin pathway and promotes EMT-like phenotype in trastuzumab-resistant HER2-overexpressing breast cancer cells.Mol Cancer Res. 10 (12): 1597-606. |
QQ:1749072012