Rabbit anti Human WNT-3a:Biotin
货号：AHP2188B
规格：50 µg
价格：4208
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：WNT-3a
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Biotin
产品介绍 |
| Rabbit anti Human RIP2 antibodyrecognizes Human Receptor-interacting serine/threonine-protein kinase 2, also known as RIP2, RIPK2, RIP-like-interacting CLARP kinase or CARD-containing interleukin-1 beta-converting enzyme-associated kinase. RIP2 is a 403 amino acid ~60 kDa cytoplasmic protein belonging to the protein kinase superfamily.RIP2 is a kinase that regulates TNF and Fas induced-apoptosis and is involved in the generation of the pro-inflammatory cytokine IL-1beta.Over expression of RIP2 has been shown to induce apoptosis, and the activation of NF-Kappa-B and JNK. RIP2 is widely expressed in a range of human tissues. |
产品详情 |
Target Species | Human |
Product Form | Purified IgG conjugated to Biotin - lyophilized |
Reconstitution | Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albuminCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. |
AntiserumPreparation | Antiserum to human WNT-3a were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Immunogen | Recombinant human WNT-3a. |
Approx. Protein Concentrations | IgG concentration 0.1mg/ml after reconstitution |
Regulatory | For research purposes only |
Guarantee | Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date. |
存储条件 |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.25 | 1.0ug/ml |
Western Blotting | √ | 0.1 | 0.2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- ELISA
- AHP2188B may be used in a direct ELISA or as the detection antibody in a sandwich ELISA with (AHP2188) as the capture reagent.
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文献 |
| 1.Lai, Y.H.et al.(2011) SOX4 interacts with plakoglobin in a Wnt3a-dependent manner in prostate cancer cells.BMC Cell Biol. 12: 50.2.Yan, L.et al.(2011) Activation of the canonical Wnt/β-catenin pathway in ATF3-induced mammary tumors.PLoS One. 6:e16515. |
技术参数 产品应用 E； WB
产品类型 Polyclonal Antibody
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