| 产品介绍 | | |
| Rabbit anti Human M-CSF antibodyrecognizes human M-CSF (Macrophage Colony-Stimulating Factor), a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor), and facilitates haematopoietic recovery following bone marrow transplantation. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - lyophilized |
| Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide |
Antiserum Preparation | Antiserum to human M-CSF were raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
| Carrier Free | Yes |
Immunogen | Recombinant human M-CSF |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml after reconstitution |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 0.5 | 2.0 ug/ml |
| Functional Assays | √ | 0.02 | 0.03 ug/ml |
| Immunohistology - Paraffin1 | √ | | |
| Western Blotting | √ | 0.1 | 0.2 ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Western BlottingThis antibody may be used under either reducing or non-reducing western blotting conditions withPHP220as the positive control. |
| 数据 |
| Formalin fixed, paraffin embedded human cervical squamous cell carcinoma stained with Rabbit anti Human M-CSF (AHP1915) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0).Formalin fixed, paraffin embedded human cervical squamous cell carcinoma stained with Rabbit anti Human M-CSF (AHP1915) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0). |
| 相关试剂 |
| Description | Product Code | Applications | Pack Size |
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB | 0.5 mL |
| Antigen Retrieval Buffer, pH8.0 | BUF025A | IHC(P) | 500 mL |
QQ:1749072012