Goat anti Human CD318 (C-Terminal)
货号:AHP1567
规格:0.1 mg
价格:4463
产品类型:免疫组化一抗
品牌:Bio-Rad
抗原:CD318 (C-Terminal)
物种:人
宿主:山羊
抗体亚型:IgG
荧光染料:Purified
产品介绍 | | |
Goat anti Human CD318 antibodyrecognises an epitope within the C-terminal (CT) region of isoform 1 of human CD318, otherwise known as CDCP1 (CUB domain-containing protein 1), a novel type I transmembrane protein expressed on the surface of haematopoietic stem cell subsets, epithelial cells and breast carcinoma, and up-regulated in metastatic lung and colorectal tumors.Expression of CD318 on CD34+CD133+leukaemic blasts from patients with AML (acute myeloid leukaemia) and CML (chronic myeloid leukaemia), suggesting a role for CD318 as a novel leukaemic diagnostic marker (Uhr 2013). |
产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human CD318 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Immunogen | Peptide sequence C-PLLNTQEPMEPAE corresponding to the C-Terminal region of CD318 (NP_073753.3). |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/16000 |
Western Blotting | √ | 1.0 | 3.0ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1567 detects a band of approximately 90kDa in human colon cell lysates.
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二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Rabbit anti Goat IgG (Fc):FITC | STAR122F | Flow | 1 mg |
Rabbit anti Goat IgG (Fc):HRP | STAR122P | C;E;WB | 1 mg |
数据 |
Western blotting analysis of human colon lysate (35μg protein in RIPA buffer) probed with Goat anti Human CD318 antibody (AHP1567) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence. |
技术参数 产品应用 ELISA;WB
产品类型 Polyclonal Antibody
推荐用于 ELISA;WB
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