| 产品介绍 | | |
| Rabbit anti Rat alpha synuclein (pSer129) antibodyrecognizes alpha synuclein when phosphorylated at serine 129. Alpha synuclein is a 140 amino acid, ~15 kDa protein seen predominantly in the neocortex, hippocampus, substantia nigra, thalamus and cerebellum. Its normal cellular function has not been definitively determined, but it is thought to be involved in the regulation of neuronal plasticity, dopamine neurotransmission and other membrane-associated processes at the presynaptic terminal.Alpha synuclein is normally a soluble, unfolded protein, but it can aggregate at high concentrations to form insoluble fibrils which, in association with other proteins such as ubiquitin, neurofilament protein and alpha B crystalline, form Lewy bodies. Lewy bodies are a hallmark pathological feature of Parkinson's disease and other neurodegenerative diseases. Alpha synuclein has several possible phosphorylation sites, serine 129 in particular can be phosphorylated by G-protein-coupled receptor kinases and this phosphorylation promotes formation of alpha synuclein filaments, with hyperphosphorylation of serine129 seen in Lewy bodies. |
| 产品详情 | | |
Target Species | Rat |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to Alpha Synulein (pSer129) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | 10mM Hepes pH7.5, 150mM NaCl |
Preservative Stabilisers | 0.09%Sodium Azide (NaN3)50%Glycerol0.01%Bovine Serum Albumin |
Immunogen | Synthetic phosphopeptide corresponding to amino acid sequence surrounding phosphorylated serine 129 of Alpha Synuclein. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | | 1/1000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1537 detects a band of approximately 15kDa in rat cortex lysate.
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| 数据 |
| Western blot of rat cortex lysate showing phospho-specific labeling of the ~15 kDa alpha synuclein protein phosphorylated at Ser129using Rabbit anti Mouse alpha synuclein (pSer129) antibody (AHP1537). |
| 文献 |
| 1. Smith, W. W.et al(2005) Alpha-Synuclein Phosphorylation Enhances Eosinophilic Cytoplasmic Inclusion Formation in SH-SY5Y Cells.J. Neurosci. 25: 5544-52. |
QQ:1749072012