| 产品介绍 | | |
| Rabbit anti Rat GSK3 beta (pSer9) antibodyrecognizes GSK3 beta, also known as glycogen synthase kinase-3 beta (GSK-3 beta), factor A (FA) and serine/threonine-protein kinase GSK3B, when phosphorylated at serine 9. GSK3 is a ~46 kDa member of the serine/threonine protein kinase family. Two isforms of GSK3 exist, alpha and beta. GSK3 beta is expressed in the testis, thymus, prostate, and ovary as well as being expressed at low levels in lung, brain and kidney tissue.GSK3 beta is involved in many signalling pathways including energy metabolism, body pattern formation and microtubule growth and stability (which aids axon growth). GSK3 beta is also thought to be involved in oncogenesis and the hormonal control of several regulatory proteins including glycogen synthase.Phosphorylation of serine-9 inhibits GSK3 beta function. |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | Purified IgG conjugated to Biotin - liquid |
| Antiserum Preparation | Antisera to rat GSK3 beta (pSer9) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography. |
| Buffer Solution | 10mM Hepes pH7.5 |
| Preservative Stabilisers | 0.09%Sodium Azide (NaN3)50%Glycerol0.01%Bovine Serum Albumin |
| Immunogen | Phosphopeptide corresponding to amino acid sequence surrounding phoshorylated seine 9 of rat GSK3 beta. |
| Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.Green, J.B. (2004) Lkb1 and GSK3-beta: kinases at the center and poles of the action.Cell Cycle. 3 (1): 12-4.2.Papkoff, J.et al. (1998) WNT-1 and HGF regulate GSK3 beta activity and beta-catenin signaling in mammary epithelial cells.Biochem. Biophys. Res. Commun. 247:851-8.3.Stambolic, V.et al. (1994) Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation.Biochem, J. 303:701-4.4.Garrido, J.et al. (2007) GSK3 alpha and GSK3 beta are necessary for axon formation.FEBS Lett. 581:1579-86. |
QQ:1749072012