Goat anti Human Ku80 antibody
货号：AHP1391
规格：0.1 mg
价格：5083
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Ku80
物种：人
宿主：山羊
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Goat anti Human Ku80 antibodyrecognizes the 80 kDa subunit of human Ku protein, an evolutionarily conserved nuclear ATP-dependent DNA helicase, involved in a major proportion of DNA repair and in V(D)J recombination.The Ku protein, originally described as an autoantigen, exists as a tightly associated heterodimer consisting of a 70 kDa (Ku70) and 80 kDa (Ku80) subunit which binds to DNA double-strand break ends (DSB). DNA bound Ku recruits the large catalytic subunit DNA-PKcs to form the DNA-dependent protein kinase complex DNA-PK, facilitating DNA repair by the non-homologous end-joining (NHEJ) pathway. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human Ku80 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Immunogen | Peptide sequence C-SLAKKDEKTDTLED from the internal region of Ku80 (NP_066964.1). |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/16,000 |
Western Blotting | √ | 0.1 | 0.3ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1391 detects a band of approximately 80-85kDa in HeLa cell lysates. An additional 75kDa band of unknown origin may be observed.
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| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | Flow | 1 mg |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C;E;WB | 1 mg |
| 数据 |
Western blotting analysis of HeLa cell lysate (35μg protein in RIPA buffer) probed using Goat anti Human Ku80 antibody (AHP1391) at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence. |
| 文献 |
| 1. Koike, M. (2002) Dimerization, translocation and localization of Ku70 and Ku80 proteins.J Radiat Res. 43 (3): 223-36.2. Dedieu, S.et al.(2010) The cytoprotective drug amifostine modifies both expression and activity of the pro-angiogenic factor VEGF-A.BMC Med. 8:19.3. Slupianek, A.et al.(2011) BCR/ABL Stimulates WRN to Promote Survival and Genomic Instability.Cancer Res. 71: 842-51. |
技术参数 产品应用 ELISA;WB
产品类型 Polyclonal Antibody
推荐用于 ELISA;WB
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