| 产品介绍 | | |
| Rabbit anti Slug antibodydetects Slug, a member of the Snail family of zinc finger transcription factors, whose expression is induced by FGF, BMP, and TGF-β. Slug is a ~29 kDa transcriptional repressor, which functions by binding to E-box motifs and inhibiting gene expression by recruiting histone deacetylase-1 (HDAC-1).Slug controls the expression of a wide variety of genes includingTP53, BRCA2, PUMandCDH1, and has important roles in apoptosis and epithelial-mesenchymal transition. It is also thought to function in neural crest development.Slug expression in breast, oesophageal, and colorectal carcinomas has been correlated with poor prognosis for survival. Rabbit anti Slug antibody is not cross-reactive with Snail. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to human Slug were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | 13 amino acid peptide sequence near the centre of human Slug. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | 0.5 | 2.0ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1186 detects a band of approximately 30kDa in 293 cell lysate.
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| 文献 |
| 1. Tripathi, M.K.et al.(2005) Regulation of BRCA2 gene expression by the SLUG repressor protein in human breast cells.J Biol Chem. 280 (17): 17163-71. |
QQ:1749072012