Rabbit anti Human CD254 antibody
货号：AHP1209B
规格：50 µg
价格：4208
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：CD254
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Biotin
| 产品介绍 | | |
| Rabbit anti Human interleukin-32 antibodyrecognises IL-32, a ~26 kDa cytokine, thought to play a role in innate and adaptive immune responses. IL-32 is produced by mitogen-activated lymphocytes, interferon-gamma activated epithelial cells and IL-12, IL-18, IL-32-activated NK cells. It is expressed at elevated levels after the activation of T-cells by mitogens or the activation of NK cells by IL-2. IL-32 activates NF-kappa-B and p38 MAPK cytokine signal pathways. TNF-alpha, IL-1beta, IL-6 and 2 C-X-C chemokine family members, all involved in autoimmune and inflammatory diseases, including rheumatoid arthritis are induced by IL-32. |
| 产品详情 | | |
| Target Species | Human |
| Reconstitution | Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. |
| Antiserum Preparation | Antisera to human CD254 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
| Product Form | Purified IgG conjugated to Biotin - lyophilized |
| Buffer Solution | Phosphate buffered saline |
| Reconstitution | Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. |
| Carrier Free | Yes |
| Immunogen | Recombinant human CD254. |
| Approx. Protein Concentrations | None present |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 0.1 | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Boyle, W.J.et al.(2003) Osteoclast differentiation and activation.Nature. 423 (6937): 337-42. |
技术参数 产品应用 elisa；wb
产品类型 Polyclonal Antibody
推荐用于 elisa；wb
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