| 产品介绍 | | |
| Rabbit anti IRF7 antibodydetects an epitope within the C-terminal (CT) of human interferon regulatory factor 7 (IRF7), a ~54 kDa transcriptional activator of type I interferons (IFNalpha/beta) in response to local and systemic viral infection. IRF7 is mostly expressed by plasmacytoid dendritic cells (pDC), which differentiate in response to IFNs and virus infection. This then stabilizes the IRF7 protein, in turn leading to increased IFN production.Multiple IRF7 transcript variants have been identified. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to human IRF7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | Pack Size: 0.1 mg Pack Size: 50 µg <0.1% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | 14 amino acid peptide sequence near the carboxy terminus of human IRF7. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | 0.5Pack Size: 0.1 mg1.0Pack Size: 50 µg | 1.0ug/mlPack Size: 0.1 mg2.0ug/mlPack Size: 50 µg |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1180 detects a band of approximately 51kDa in 293 whole cell lysate.
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| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
| Immunohistochemical staining of mouse spleen with Rabbit anti Human IRF7 (AHP1180) |
| 文献 |
| References for IRF7 antibody1. Rautela, J.et al.(2015) Loss of Host Type-I IFN Signaling Accelerates Metastasis and Impairs NK-cell Antitumor Function in Multiple Models of Breast Cancer.Cancer Immunol Res. 3 (11): 1207-17.2. Saitoh, S.I.et al.(2017) TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells.Nat Commun. 8 (1): 1592.Further Reading1. Ning, S.et al.(2005) Regulation of the transcriptional activity of the IRF7 promoter by a pathway independent of interferon signaling.J Biol Chem. 280 (13): 12262-70.2. Prakash, A. & Levy, D.E. (2006) Regulation of IRF7 through cell type-specific protein stability.Biochem Biophys Res Commun. 342 (1): 50-6. |
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