| 产品介绍 | | |
| Rabbit anti Human clusterin antibodyrecognizes human clusterin, also known as apolipoprotein J, Ku70-binding protein 1 or TRPM-2. Clusterin is a ubiquitous glycoprotein which exists in at least two distinct isoforms; a proapoptotic nuclear form, of ~49 kDa, and a prosurvival secretory form with a molecular mass of 75-80 kDa. Clusterin is implicated in several diverse physiological processes including sperm maturation, cell differentiation, and apoptosis.Clusterin upregulation has been reported in various human malignancies, including bladder, prostate, and breast tumors.Following specific stress induced injury, secretory clusterin acts as a molecular chaperone, scavenging denatured proteins outside cells. Clusterin upregulated in Alzheimer’s disease (Minerset al.2017), retinitis pigmentosa (Jomaryet al1993), glomerulonephritis, atherosclerosis and myocardial infarction (Rosenberget al.2002). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - lyophilized |
| Antiserum Preparation | Antisera to Clusterin were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
| Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.02%Sodium Azide (NaN3) |
| Immunogen | Recombinant human clusterin isoform 1. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 0.5 | 1.0ug/ml |
| Immunohistology - Paraffin1 | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
QQ:1749072012