| 产品介绍 | | |
| Rabbit anti Human IL-23 antibodyrecognizes the C-terminal (CT) domain of human interleukin-23 (IL-23), a ~21 kDa heterodimeric cytokine structurally related to IL-12. IL-23 is composed of a p19 subunit encoded by the IL23A gene, and a p40 subunit of interleukin-12 (IL12B). IL-23 is expressed mainly by activated macrophages and dendritic cells, and plays an important role in bridging innate and adaptive responses.IL-23 activates a subset of T cells called Th17 cells, which are characterised by the production of interleukin-17. The IL-23/IL-17 pathway is essential for the induction and maintenance of brain and joint inflammatory autoimmune diseases, including Guillain Barre Syndrome, Multiple sclerosis and rheumatoid arthritis. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to human IL-23 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | A 14 amino acid peptide from near the carboxy-terminus of human IL-23. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Frozen | √ | | 10ug/ml |
Western Blotting | √ | 1 | 2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Raji cells.
- Western Blotting
- AHP1173 detects a band of approximately 24kDa in Raji cell lysates
|
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| 数据 |
Western blot analysis of whole cell lysate form Raji, human Burkitt's lymphoma derived cells probed with Rabbit anti Human Interleukin-23 (AHP1173) in the absence (A) or presence (B) of blocking peptide. |
| 文献 |
| 1. Kikly, K.et al.(2006) The IL-23/Th(17) axis: therapeutic targets for autoimmune inflammation.Curr Opin Immunol. 18 (6): 670-5.2. Hu, W.et al.(2006) Interleukin 23 in acute inflammatory demyelination of the peripheral nerve.Arch Neurol. 63 (6): 858-64.3. Langrish, C.L.et al.(2004) IL-12 and IL-23: master regulators of innate and adaptive immunity.Immunol Rev. 202: 96-105.4. Hunter, C.A. (2005) New IL-12-family members: IL-23 and IL-27, cytokines with divergent functions.Nat Rev Immunol. 5 (7): 521-31. |
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