| 产品介绍 | | |
| Rabbit anti Human DKK1 antibodyrecognises human Dickkopf (Xenopus laevis) homolog 1 (DKK1), a 35 kDa to 50 kDa member of the dickkopf family. It is expressed in the placenta, prostate and to a lesser extent, in the colon and spleen. DKK1 is secreted when expressed in 293T cells.DKK1 inhibits Wnt signalling in embryonic development, most likely acting upstream of frizzled. It is also thought to contribute to neoplastic processes, inhibit osteoblast differentiation and is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.1% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | Synthetic human DKK1 - KLH conjugated. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Paraffin1 | √ | | 10ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Uterus, endometrium.
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| 数据 |
Staining of formalin fixed, paraffin embedded human endometrium with Rabbit anti Human DKK1 (AHP1156). |
| 文献 |
| 1. Krupnik, V.E.et al.(1999) Functional and structural diversity of the human Dickkopf gene family.Gene. 238 (2): 301-13.2. Fedi, P.et al.(1999) Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling.J Biol Chem. 274 (27): 19465-72.3. Niehrs C. (2006) Function and biological roles of the Dickkopf family of Wnt modulators.Oncogene. 25:7469-81. |
QQ:1749072012