| 产品介绍 | | |
| Goat ati Human SNAP25 antibodyrecognizes human synaptosomal-associated protein, 25kD (SNAP-25), also known as Super protein (Sup) or Synaptosomal-associated 25 kDa protein. SNAP-25 is a 206 amino acid, presynaptic plasma membrane protein containning 2tSNARE coiled-coildomains and is essential for Ca2+-regulated exocytosis of neurotransmitters. SNAP-25 binds to and negatively modulate Ca2+ channels, increasing the speed of neurotransmitter release (Rettiqet al.1996) .Goat ati Human SNAP25 antibody detects an epitope within the C-terminal (CT) of human SNAP25 (aa 193-206).As a result of alternative splicing, SNAP-25 is expressed by neurons and neuroendocrine cells as two predominant isoforms; SNAP-25a and SNAP-25b. SNAP-25a is expressed early in development, whereas SNAP-25b expression is linked to synaptogenesis (Bark and Wilson 1994). Goat ati human SNAP25 antibody is expected to recognise both isoforms.SNAP-25 may be a possible therapeutic treatment target for sufferers of hyperactivity disorders (Barret al.2000), whilst SNAP-25 based peptides may be a possible treatment for involuntary muscle spasms (Hodel 1998). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to human SNAP-25 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide 0.5%Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
Immunogen | Peptide sequence C-DEANQRATKMLGSG corresponding to the C-terminus of SNAP-25 (NP_003072.2, NP_570824.1). |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/32000 |
Immunohistology - Paraffin1 | √ | 2.0 | 4.0ug/ml |
Western Blotting | √ | 0.01 | 0.03ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. Bio-Rad recommend citrate buffer, pH6.0, for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1124 detects a band of approximately 22kDa in human and mouse brain lysates. A minimum incubation time of 1 hour is recommended with this antibody.
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| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | Flow | 1 mg |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C;E;WB | 1 mg |
| 数据 |
| Immunohistochemical staining of formalin fixed, paraffin embedded Human cerebellum following heat mediated antigen retrieval in citrate buffer at pH6.0. A. Cerebellum stained using Goat anti Human SNAP25 antibody (AHP1124) at 1.0μgml-1. B. Negative control showing cerebellum staining with primary antibody omitted. |
| 文献 |
| 1. Hodel, A. (1998) SNAP-25.Int J Biochem Cell Biol. 30 (10): 1069-73.2. Lazzarini, R.et al.(2019) Mesenchymal Stem Cells from Nucleus Pulposus and Neural Differentiation Potential: a Continuous Challenge.J Mol Neurosci. 67 (1): 111-124. |
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