Goat anti Rat Leptin antibody
货号：AAR40
规格：0.1 mg
价格：4063
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Leptin
物种：大鼠
宿主：山羊
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Goat anti Rat Leptin antibodyspecifically recognizes rat leptin, a major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.Leptin is a recognized marker of obesity, which signals through binding to the obesity receptor (OB-R) in the hypothalamus. Human studies have shown a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones. |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | Purified IgG - lyophilized |
| Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. |
| Antiserum Preparation | Antiserum to rat Leptin were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | None present. |
| Immunogen | Recombinant rat leptin. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml after reconstitution |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 0.5 | 2.0ug/ml |
| Functional Assays | √ | 0.1 | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 100 Tests |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C,E,WB | 100 Tests |
技术参数 产品应用 wb；elisa
产品类型 Polyclonal Antibody
推荐用于 wb；elisa
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