Rabbit anti Rat MCP-1 antibody
货号：AAR31
规格：0.1 mg
价格：4063
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：MCP-1
物种：大鼠
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti Rat MCP-1 antibodyrecognizes rat monocyte chemo attractant protein-1 (MCP-1), also known as CCL-2.Rat MCP-1 is a chemokine of approximately 12 kDa, which plays an important role in the inflammatory response of blood monocytes and tissue macrophages. |
| 产品详情 | | |
| Target Species | Rat |
| Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide |
| Antiserum Preparation | Antisera to rat MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
| Product Form | Purified IgG - liquid |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | None present |
| Immunogen | Recombinant rat MCP-1. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml after reconstitution. |
| Regulatory | For research purposes only |
| Guarantee | 6 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 0.5 | 2.0ug/ml |
| Functional Assays | √ | 4.0 | 6.0ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.Zhang, H.et al.(2016) Dorsal root ganglion infiltration by macrophages contributes to paclitaxel chemotherapy induced peripheral neuropathy.J Pain. Mar 12. pii: S1526-5900(16)00562-9. [Epub ahead of print]2.Aharon-Hananel, G.et al.(2015) Antidiabetic Effect of Interleukin-1β Antibody Therapy Through β-Cell Protection in the Cohen Diabetes-Sensitive Rat.Diabetes. 64 (5): 1780-5.3.Schreiber, R.C.et al.(2001) Monocyte chemoattractant protein (MCP)-1 is rapidly expressed by sympathetic ganglion neurons following axonal injury.Neuroreport. 12 (3): 601-6.4.Tomas-sanchez, C.et al.(2018) Prophylactic Zinc and Therapeutic Selenium Administration Increases the Antioxidant Enzyme Activity in the Rat Temporoparietal Cortex and Improves Memory after a Transient Hypoxia-Ischemia.Oxid Med Cell Longev. 2018: 9416432.5.Rong, Y.et al.(2021) Small extracellular vesicles encapsulating CCL2 from activated astrocytes induce microglial activation and neuronal apoptosis after traumatic spinal cord injury.J Neuroinflammation. 18 (1): 196. |
技术参数 产品应用 elisa；wb
产品类型 Polyclonal Antibody
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