Goat anti Mouse M-CSF antibody
货号：AAM75
规格：0.1 mg
价格：4063
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原： M-CSF
物种：小鼠
宿主：山羊
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Goat anti Mouse M-CSF antibodyrecognizes mouse M-CSF (Macrophage colony-stimulating factor), a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor). A genetic defect in M-CSF is responsible for osteopetrosis in mice (op/op), which are severely deficient in mature macrophages and have a restricted capacity for bone remodelling. |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG - lyophilized |
Antiserum Preparation | Antiserum to mouse M-CSF was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml after reconstitution |
Immunogen | Recombinant mouse M-CSF. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.5 | 2.0ug/ml |
Functional Assays | √ | 0.05 | 0.08ug/ml |
Western Blotting | √ | 0.1 | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | Flow | 1 mg |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C;E;WB | 1 mg |
| 文献 |
| 1. Djelloul, M.et al.(2016) RAE-1 expression is induced during experimental autoimmune encephalomyelitis and is correlated with microglia cell proliferation.Brain Behav Immun. 58: 209-17. |
技术参数 产品应用 ELISA;FN;WB
产品类型 Polyclonal Antibody
推荐用于 ELISA;FN;WB
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