| 产品介绍 | | |
| Rabbit anti Mouse IP-10 antibodyrecognizes interferon gamma inducible protein (IP-10), also known as CXCL10. IP-10 is expressed in a variety of tissues including liver, gut, spleen, kidney and thymus, in response to interferon gamma.IP-10 acts as a chemoattractant for monocytes, NK cells and certain T-cells. |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG - lyophilized |
Antiserum Preparation | Antisera to mouse IP-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml after reconstitution |
Immunogen | Recombinant mouse IP-10. |
Regulatory | For research purposes only |
Guarantee | 6 months from date of despatch |
Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 0.5ug/ml |
Functional Assays | √ | 0.5ug/ml | 0.9ug/ml |
| Immunohistology - Frozen | √ | | |
Western Blotting | √ | 0.1ug/ml | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.ELISAPurified rabbit anti mouse IP-10 may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with ourbiotinylated anti mouse IP-10 antibody(AAM39B) as a detection reagent. |
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| 文献 |
| 1. Zhou, J.et al.(2010) CXCR3-dependent accumulation and activation of perivascular macrophages is necessary for homeostatic arterial remodeling to hemodynamic stresses.J Exp Med. 207 (9): 1951-66. |
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