| 产品介绍 | | |
| Mouse anti Human CD142 antibody, clone TF9-10H10recognizes human CD142, also known as Tissue Factor, is the membrane receptor for coagulation factors VII and VIIa and is the cell surface initiator of coagulation. It is the major molecule of this type and is criticial for controlling hemostasis, thrombosis and inflammation.Mouse anti Human CD142 antibody, clone TF9-10H10recognizes an epitope within the extracellular domain, epitope locus I. It recognizes both the reduced and native non-reduced human and primate tissue factors. It does not inhibit coagulation or neutralize factor VII binding to CD142. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from ascites. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3)100 mM Mannitol |
| Immunogen | Denatured Tissue factor isolated from human brain by the Factor VII affinity method (Guhaet al.1986). |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunized Balb/c mice were fused with cells of the P3Ag8.653.1myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 0.5 µg/ml | 1 µg/ml |
| Immunohistology - Paraffin | √ | 10 µg/ml | 20 µg/ml |
| Western Blotting | √ | 1 µg/ml | 2 µg/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5mg |
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E,WB | 0.5mg |
| 文献 |
| 1.Morrissey, J.H.et al.(1988) Monoclonal antibody analysis of purified and cell-associated tissue factor.Thromb Res. 52 (3): 247-61.2.Contrino, J.et al.(1994)In situcharacterization of antigenic and functional tissue factor expression in human tumors utilizing monoclonal antibodies and recombinant factor VIIa as probes.Am J Pathol. 145 (6): 1315-22.3.Drake, T.A.et al.(1989) Selective cellular expression of tissue factor in human tissues. Implications for disorders of hemostasis and thrombosis.Am J Pathol. 134 (5): 1087-97.4.Stearns-Kurosawa, D.J.et al.(2006) Sepsis and pathophysiology of anthrax in a nonhuman primate model.Am J Pathol. 169 (2): 433-44. |
QQ:1749072012