| 产品介绍 | | |
| Rabbit antiSalmonellagroup antigen antibodyrecognizes aSalmonellagroup antigen.Salmonellais a genus of the familyEnterobacteriaceaepopulated by a variety of Gram negative rod-shaped bacteria, many of which are pathogenic and cause a range of diseases in humans.Salmonellaepossess 3 major surface antigens: the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in otherEnterobacteriaceae).Salmonellaealso possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A.Rabbit antiSalmonellagroup antigen antibody is polyvalent forSalmonellaO and H antigens.Rabbit antiSalmonellagroup antigen antibody is unabsorbed and may cross react with relatedEnterobacteriaceae. |
| 产品详情 | | |
Target Species | Bacterial |
Product Form | Purified IgG - liquid |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.002% Thiomersal1% Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Immunogen | Mixture ofSalmonella enteriditis,S. typhimuriumandS. heidelburg. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/200 | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
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| 数据 |
| Published customer image:Rabbit antiSalmonellagroup antigen antibody (8209-4006) used as a detection reagent in the fabrication of a biosensor for contamination detection in water samples.Image caption:Real-time sensor response obtained from chips coated with a 200 μg/mL Salmonella LPS solution applying either the (a) two-step or (b) three-step assay configurations. The two-step assay configuration (a) included running over the chip 1:1 volume mixtures of calibrators with a 3 μg/mL rabbit anti-Salmonellaantibody solution (arrow 1 to 2), followed by an Anti-Rabbit antibody solution (arrow 2 to end); while the three-step assay configuration (b) included running over the chip 1:1 volume mixtures of calibrators with a 1.5 μg/mL rabbit anti-Salmonellaantibody solution (arrow 1 to 2), followed by biotinylated Anti-Rabbit IgG antibody (arrow 2 to 3) and streptavidin (arrow 3 to end). Black line corresponds to zero calibrator, red line to a calibrator containing 500 ng/mL LPS, and blue line to signal obtained from a chip spotted with BSA and assayed as the LPS-spotted chip.From: Angelopoulou M, Tzialla K, Voulgari A, Dikeoulia M, Raptis I, Kakabakos SE, Petrou P.Rapid Detection ofSalmonella typhimuriumin Drinking Water by a White Light Reflectance Spectroscopy Immunosensor.Sensors. 2021; 21(8):2683.doi: 10.3390/s21082683This image is from an open access article distributed under terms of aCreative Commons Attribution License. |
| 文献 |
| 1. Duffy, G.et al.(2000) A membrane-immunofluorescent-viability staining technique for the detection of Salmonella spp. from fresh and processed meat samples.J Appl Microbiol. 89 (4): 587-94.2. Hunter, D.M.et al.(2010) Rapid detection and identification of bacterial pathogens by using an ATP bioluminescence immunoassay.J Food Prot. 73: 739-46.3. Ewald, M.et al.(2015) A multi-analyte biosensor for the simultaneous label-free detection of pathogens and biomarkers in point-of-need animal testing.Anal Bioanal Chem. 407 (14): 4005-13.4. Ewald Met al.(2013) A robust sensor platform for label-free detection of anti-Salmonellaantibodies using undiluted animal sera.Anal Bioanal Chem. 405 (20): 6461-9.5. Cloak, O.M.et al.(1999) Isolation and detection ofListeriaspp,Salmonellaspp andYersiniaspp using a simultaneous enrichment step followed by a surface adhesion immunofluorescent technique.J Microbiol Methods. 39 (1): 33-43. |
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