产品介绍 |
| Mouse anti Human cytokeratin 19 antibody, clone A53-B/A2recognizes the rod domain (aa 312-335) of human cytokeratin 19 (Böttger;et al.1995) also known as keratin 19 encoded by the KRT19 gene. Cytokeratin 19 is a 400 amino acid intermediate filament protein lacking a C-terminal tail domain, in contrast to all other intermediate filament proteins.Cytokeratin 19 expression is observed in striated muscle where it is involved in forming the association between the contractile apparatus and dystrophin (Stoneet al.2005). Expression is also seen in many ductal and glandular cells together with a restricted set of normal and neoplasic epithelial cells.Mouse anti Human cytokeratin 19 antibody, clone A53-B/A2 detects a band of ~45 kDa in western blotting using an A-549 human alveolar adenocarcinoma cell line lysate. |
产品详情 |
Target Species | Human |
Western Blotting | In Western blotting 40 kD and 19 kD bands are observed. |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09%Sodium Azide (NaN3) |
Carrier Free | Yes |
Immunogen | Human breast cancer cell lineMCF-7. |
Approx. Protein Concentrations | 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Acknowledgements | PrecisionAb is a trademark of Bio-Rad Laboratories |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
Immunofluorescence | √ | | |
Immunohistology-Paraffin1 | √ | 1/100 | 1/200 |
Western Blotting | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Clickhereto learn how we validate our PrecisionAb range.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.- Histology Positive Control Tissue
- Appendix
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文献 |
| 1.Karsten, U.et al.(1985) Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion.Eur J Cancer Clin Oncol. 21 (6): 733-40.2.Kasper, M.et al.(1987) Histological evaluation of three new monoclonal anti-cytokeratin antibodies. 1. Normal tissues.Eur J Cancer Clin Oncol. 23 (2): 137-47.3.Goletz, S.et al.(1997) Novel alphaGalNAc containing glycans on cytokeratins are recognized invitro by galectins with type II carbohydrate recognition domains.J Cell Sci. 110 ( Pt 14): 1585-96.4.de Neergaard, M.et al.(2010) Epithelial-stromal interaction 1 (EPSTI1) substitutes for peritumoral fibroblasts in the tumor microenvironment.Am J Pathol. 176: 1229-40.5.Ehlicke, F.et al.(2010) Intervertebral disc regeneration: influence of growth factors on differentiation of human mesenchymal stem cells (hMSC).Int J Artif Organs. 33 (4): 244-52.6.Johnston, R.L.et al.(2016) High content screening application for cell-type specific behaviour in heterogeneous primary breast epithelial subpopulations.Breast Cancer Res. 18 (1): 18.7.Orafa, Z.et al. (2022) Quantitative CK19 biomarker detection in breast cancer cell linesJ Med and Life 15 (2): 188-95. |
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