产品介绍 |
| Mouse anti Human Insulin/Proinsulin antibody, clone D6C4 (5E4/3), recognizes both the pro and mature forms ofhuman insulinbut does not react with free C-peptide. |
产品详情 |
Target Species | Human |
Species Cross-Reactivity | Target Species | Cross Reactivity |
| Mouse | √ |
| Rat | √ |
| Bovine | √ |
| Pig | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Immunogen | Recombinant human insulin. |
Affinity | 8.1 x 108M-1 |
Approx. Protein Concentrations | 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
Immunohistology - Frozen | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
可用二抗 |
Description | Product Code | Applications | Pack Size |
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E WB | 0.5 mg |
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) | STAR117D488GA | F IF | 0.1 mg |
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) | STAR117D550 | F IF WB | 0.1 mg |
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) | STAR117D650 | F IF | 0.1 mg |
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) | STAR117D680GA | F WB | 0.1 mg |
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | STAR117D800GA | F IF WB | 0.1 mg |
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | C E WB | 0.5 mg |
相关产品 |
Description | Product Code | Applications | Pack Size |
Mouse anti Human Insulin/Proinsulin | 5330-3369 | C E P | 0.2 mg |
文献 |
| References for Insulin/Proinsulin antibody1.Coughlan, M.T.et al.(2011) Advanced Glycation End Products Are Direct Modulators of {beta}-Cell Function.Diabetes. 60: 2523-32.2.Briand, O.et al.(2012) The Nuclear Orphan Receptor Nur77 Is a Lipotoxicity Sensor Regulating Glucose-Induced Insulin Secretion in Pancreatic β-Cells.Mol Endocrinol. 26: 399-413.3.Park, J.et al.(2012) Application of a new microcantilever biosensor resonating at the air-liquid interface for direct insulin detection and continuous monitoring of enzymatic reactions.Lab Chip. 12 (20): 4115-9.4.Gargani, S.et al.(2013) Adaptive changes of human islets to an obesogenic environment in the mouse.Diabetologia. 56 (2): 350-8. |
QQ:1749072012