| 产品介绍 | | |
| Native Rat collagen Iis purified rat collagen I derived from tail tendon tissue. |
| 产品详情 | | |
Target Species | Rat |
Product Form | Purified protein from rat tail tendon - lyophilized |
Preparation | Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I was purified by using differential salt precipitation. |
Buffer Solution | Essentially salt free |
Preservative Stabilisers | None present. |
Reconstitution | Reconstitute in ice cold 0.5 M acetic acid, pH 2.5. Solubility - 10 mg/ml. |
Purity | SDS PAGE: >90% Rat collagen type III 10% Rat collagen, other types＜1% Non-collagen proteins＜0.5% |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| Store at -20℃only.Storage in frost-free freezers is not recommended.Do not freeze after reconstitution. Stable for 1-2 months at 4ºC. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 文献 |
| 1. Miller, E.J. & Gay, S. (1982) Collagen: an overview.Methods Enzymol. 82 Pt A: 3-32. |
QQ:1749072012